Applications of Gel Permeation Chromatography: Gel permeation chromatography is also called gel filtration or size exclusion chromatography. It is a separation technique in which the separation is based on the analyte molecular sizes since the gel behaves like a molecular sieve. In-gel permeation. chromatography, the stationary phase is a porous matrix made up of compounds like; cross-linked polystyrene, dextran, polyacrylamide gels, agarose gels, etc. The molecules in the sample are pumped through specialized columns containing such microporous packing material (gel). The flow of the mobile phase causes larger molecules to pass through the column unhindered, without penetrating the gel matrix whereas, smaller molecules will be retarded according to their penetration on the gel.
Applications of Gel Permeation Chromatography
- The gel permeation chromatography technique is used for the separation of proteins, polysaccharides, enzymes, and synthetic polymers.
- Can be used to determine the quaternary structure of purified proteins.
- Separation of sugar, proteins, peptides, rubbers, and others based on their size.
- There is no sample loss. An only a small amount of mobile phase is required.
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