Aim: To prepare and submit compound sodium chloride solution (Ringer’s solution).
Requirement: Sodium chloride, Potassium chloride, Calcium chloride hydrated, Purified water beaker, Glass rod, Measuring cylinder, etc.
Theory: Ringer’s solution is a solution of several salts dissolved in water to create an isotonic solution relative to the body fluids of an animal. Ringer’s solution contains sodium chloride, potassium chloride, and calcium chloride. It is used as a fluid and electrolyte replenisher to irrigate the animal tissues while experimentation.
Formula:
| Ingredients | Standard formula |
| Sodium chloride | 8.6 g |
| Potassium chloride | 0.3 g |
| Calcium chloride hydrated | 0.33 g |
| Purified water q.s. | 100 ml |
Procedure:
Depending upon the quantity of preparation to be submitted, the working formula is calculated.
- Dissolve sodium chloride, potassium chloride, and calcium chloride hydrated in a sufficient quantity of purified water.
- Add a sufficient amount of purified water to produce the required volume.
Category: Fluid and electrolyte replenisher.
Storage: Store in a well-closed container. Always use a fresh solution.
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