Assay of Adrenaline

Molecular Formula: C₉H₁₃NO₃  

Molecular Weight: 183.2 

In general, the reaction taking place between a primary amine and perchloric acid may be expressed as follows: 

R-NH₂ + HClO₄ → [R-NH₃]⁺ + ClO⁻₄ 

Material Required: Adrenaline 0.2 g; glacial acetic acid: 50 mL; 0.1 N perchloric acid and crystal violet or oracet blue B solution. 

Procedure: Weigh accurately about 0.3 g of adrenaline into a 250 mL conical flask; add glacial acetic acid (50 mL), and warm gently, if necessary. Cool and titrate with 0.1 N perchloric acid using crystal violet or oracet blue B as an indicator. 

Reaction:

Factor Calculation: 

1 mole of perchloric acid ≅ 1 mole of adrenaline 

1000 mL of 1N HClO4 ≅ 183.2 g of C₉H₁₃NO₃  

1 mL of 0.1N HClO4 ≅ 0.01832 g of C₉H₁₃NO₃  

Make sure you also check our other amazing Article on : Examples of Non Aqueous Solvents