Assay of Methacholine Chloride

Molecular formula: C8H18ClNO2   

Molecular weight: 195.69 

Materials Required: Methacholine chloride: 0.4 g; glacial acetic acid: 50 mL; mercuric acetate solution: 10 mL; 0.1 N perchloric acid and crystal violet solution. 

Procedure: Weigh accurately about 0.4 g, previously dried and stored in a vacuum desiccator, and dissolve in 50 mL of glacial acetic acid, add 10 mL of mercuric acetate solution, and titrate with 0.1 N perchloric acid using one drop of crystal violet solution as an indicator until the color changes to blue-green color. Perform a blank determination and make any necessary corrections.


Assay of Methacholine Chloride

Factor Calculation: 

1 mole of HClO4 ≅ 1 mole of methacholine chloride 

1000 mL of 1N HClO4 ≅ 195.69 g of C8H18ClNO2 

1 mL of 0.1 N HClO4 ≅ 0.01956 g of C8H18ClNO2 

Make sure you also check our other amazing Article on : Assay of Sodium Benzoate
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