Assay of Amitriptyline Hydrochloride

Molecular formula: C20H23N⋅HCl 

Molecular Weight: 313.86 

Materials Required: Amitriptyline hydrochloride: 1.0 g; glacial acetic acid: 50 mL; mercuric acetate: 10 mL; crystal violet; 0.1 N perchloric acid. 

Procedure: Weigh accurately about 1.0 g of sample and dissolve it in 50 mL of glacial acetic acid, warm slightly, if necessary, to affect the solution. Cool, add 10 mL of mercuric acetate solution, and titrate with 0.1 N perchloric acid using two drops of crystal violet solution as an indicator until the color changes to green color. Perform a blank determination and make any necessary corrections. 


Assay of Amitriptyline Hydrochloride

(CH3COO)2 Hg + 2Cl → HgCl2 + 2CH3COO 


Factor Calculation: 

1 Mole of HClO4 ≅ 1 Mole of Amitriptyline hydrochloride 

1000 mL of 1M HClO4 ≅ 313.86 g of C20H23N⋅HCl 

1 mL of 0.1N HClO4 ≅ 0.03138 g of C20H23N⋅HCl 

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