Assay of Amitriptyline Hydrochloride

Molecular formula: C₂₀H₂₃N⋅HCl 

Molecular Weight: 313.86 

Materials Required: Amitriptyline hydrochloride: 1.0 g; glacial acetic acid: 50 mL; mercuric acetate: 10 mL; crystal violet; 0.1 N perchloric acid. 

Procedure: Weigh accurately about 1.0 g of sample and dissolve it in 50 mL of glacial acetic acid, warm slightly, if necessary, to affect the solution. Cool, add 10 mL of mercuric acetate solution, and titrate with 0.1 N perchloric acid using two drops of crystal violet solution as an indicator until the color changes to green color. Perform a blank determination and make any necessary corrections. 

Reaction:

(CH₃COO)₂ Hg + 2Cl⁻ → HgCl₂ + 2CH₃COO⁻ 

2CH₃COOH₂⁺ + 2CH₃COO⁻ ⇌ 4CH₃COOH 

Factor Calculation: 

1 Mole of HClO₄ ≅ 1 Mole of Amitriptyline hydrochloride 

1000 mL of 1M HClO₄ ≅ 313.86 g of C₂₀H₂₃N⋅HCl 

1 mL of 0.1N HClO₄ ≅ 0.03138 g of C₂₀H₂₃N⋅HCl 

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