Assay of Probenecid

Molecular Formula: C13H19NO4

Molecular Weight: 285.36 

Assay: Weigh accurately 0.5 g of probenecid sample in 100 mL of (95%) ethanol. Shake well and heat gently in a water bath if necessary to dissolve the drug. Cool and titrate with  0.1M sodium hydroxide using bromothymol blue as an indicator. The endpoint will be yellow to blue. Carry out a blank titration. 


Factor Calculation: 

1 Mole of NaOH ≅ 1 Mole of probenecid 

1000 mL of 1M NaOH ≅ 285.36 g of C13H19NO4S   

1 mL of 0.1M NaOH ≅ 0.02854 g of C13H19NO4

Make sure you also check our other amazing Article on : Assay of Sodium Carbonate
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